Clinical study on the ocular penetration of intravenous ciprofloxacin in open globe injury of human eyes

Abstract

Objective To assess the ocular penetration of ciprofloxacin in open globe injury of human eyes after a single dose intravenous administration.Methods In this prospective study,16 patients with non-purulent open globe injury were studied.The normal control group included 12 patients underwent cataract surgery.All the patients received continuously intravenous infusion of 200mg ciprofloxacin before surgery.The aqueous samples were obtained in both group and in open globe injury group vitreous humour samples were also obtained.Drug concentrations were measured using high-pressure liquid chromatography analysis and protein concentration of aqueous humor was measured by biuret conorimetric method simultaneously.Results 1.The protein concentration of aqueous humor in open globe injury group(6.482±2.981g/l)were significantly higher than that of the control group(0.067±0.037g/l),(P 0.05).3.The vitreous concentration of ciprofloxacin in open globe injury group was 0.082±0.022μg/ml.The vitreous levels of ciprofloxacin in open globe injury group could not exceed the 90% minimum inhibitory concentration(MIC90)of common ocular pathogens causing endophthalmitis.Conclusions In the open-globe injured eye the blood-ocular barrier was damaged,but the ocular penetration of ciprofloxacin didn't enhance.The levels of intraocular ciprofloxacin after a single dose of intravenous administration couldn't reach effective therapeutic concentration. Key words: Ciprofloxacin ; Continuously intravenous infusion ; Ocular penetration ; Open globe injury ;