Clinical study of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry technology in detecting the anxiety of patients with breast cancer

Abstract

Objective To investigate the incidence of anxiety in patients with breast cancer,and further explore the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI) technology's clinical value in the diagnosis of anxiety. Methods 121 breast cancer patients were selected as research objects by using convenience sampling method. Self-rating anxiety scale (SAS) was used to evaluate the anxiety on the first day of admission and SELDI was used on the next day to detect the serum from patients' fasting venous blood sample got in the morning after their consent, then anxiety-related protein fingerprints spectrums were selected. The SAS score and SELDI result were finally compared and analyzed. Results SAS score in 121 patients was 53.45±9.78, anxiety occurred in 63 cases (52.07 %). Established the diagnostic model between 15 000+H and 16 800+H protein fingerprints abundance ≥5 %, that was to say, abundance ≥5 % was judged as positive, otherwise negative. On this basis, patients can accurately be distinguished between anxious group and non-anxious group. Correct rate (total coincidence rate) was 91.74 %, specificity and sensitivity respectively were 89.66 % (52/58) and 93.65 % (59/63), the positive predictive value was 90.77 % (59/65), and negative predictive value was 92.86 % (52/56). Conclusions Breast cancer patients have a high incidence of anxiety. SELDI technology has showed high sensitivity and specificity in anxiety detecting. As an objective assessment tool, it could have better prospects for clinical use. Key words: Breast neoplasms; Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry; Anxiety; Self-rating anxiety scale