Clinical study of miRNA-22 level in cerebrospinal fluid using in bacterial meningitis early diagnosing and prediction of therapeutic effect

Abstract

Objective To research the clinical value of miRNA-22 level in cerebrospinal fluid (CSF) in bacterial meningitis early diagnosing and prediction of therapeutic effect. Methods Eighty-two children with meningitis admitted to The Maternal and Child Care Service Center of Nanhai District from January 2015 to March 2018 and 44 children with meningitis admitted to the Second People’s Hospital of Foshan City at the same time were selected. They were divided into bacterial meningitis group (n=64), viral meningitis group (n=44), tuberculous meningitis group (n=18) according to the type of etiology. The copies of miRNA-22 in CSF of three groups were compared before and after treatment. The therapeutic effect of bacterial meningitis in children was divided into satisfying group (28 cases), general group (19 cases) and indisposed group (17 cases) under the conventional standard treatment. The levels of miRNA-22 in CSF were compared before and after treatment at 1, 3 and 5 d in order to evaluate the relationship between the level and variation tendency of miRNA-22 and the therapeutic effect of the disease. Results Compared with viral meningitis group and tubercular meningitis group, the copy number of miRNA-22 in bacterial meningitis group is significantly higher before treatment (P 0.05), but the worse the curative effect, the higher the level of miRNA-22 at admission. the copy number of miRNA-22 in CSF of bacterial meningitis group with satisfactory therapeutic effect was significantly lower than general group and indisposed group. Conclusion The level of miRNA-22 copies in CSF of children with meningitis has certain reference value for early distinguishing the types of pathogenic bacteria in children with meningitis. The number of miRNA-22 copies in CSF of children with bacterial meningitis is significantly increased, and the number of miRNA-22 copies may be correlated with the anticipated therapeutic effect of children with bacterial meningitis. Key words: miRNA-22; Bacterial meningitis; Early diagnosing; Children