Abstract
目的 探讨外周血白细胞巨细胞病毒(cytomegalovirus,CMV)抗原(即刻早期抗原和早期抗原)检测、唾液聚合酶链反应(PCR)检测CMV-DNA和血清学检测CMV-IgM三种实验方法在诊断和监测新生儿先天性CMV感染中的作用.方法对98例患儿之母在孕期血CMV-IgM阳性的高危新生儿出生后14 d内检查血CMV-IgM、外周血白细胞CMV抗原和唾液PCR-CMV-DNA,比较三种方法的检测结果,并对新生儿进行临床观察和随访.结果(1)98例中,48例诊断先天性CMV感染.症状性感染7例,无症状性感染41例;无一例CMV-IgM阳性.症状性感染组CMV抗原、PCR阳性检出率分别为7/7、5/7;无症状性感染组阳性检出率分别为71%(29/41)、46%(19/41).CMV抗原检测法、PCR检测法的诊断敏感性分别为75%、54%.症状性感染儿每5万白细胞中CMV阳性细胞数16～52个,无症状性感染儿3～31个,症状性感染儿CMV抗原指数水平显著高于无症状感染儿(P＜0.05).(2)随访10例患儿,2例症状性感染儿CMV抗原和PCR转阴,症状消失;1例CMV肝炎治疗后CMV抗原和PCR检测仍阳性,最后发展为肝硬化.7例无症状性感染儿,CMV抗原在6个月内转阴或减少至低水平;2例伴唾液PCR阳性,复查时1例转阴,1例仍阳性.结论CMV抗原检测法具有早期、敏感、量化的特点,PCR法能发现潜伏性感染,两者联合应用能提高诊断阳性率,对治疗和随访患儿提供有效帮助;CMV-IgM法对先天性CMV感染的早期诊断的敏感性低。
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
