Clinical study of CD64 combined with chemokine in neonatal sepsis

Abstract

Objective To investigate the value of neutrophil CD64 combined with monocyte chemotactic protein 1 (MCP-1), interleukin-8 (IL-8) and interferon-inducible protein-10 (IP-10) in the clinical diagnosis of neonatal sepsis. Method Cases of neonatal sepsis from March 2015 to June 2016 in the department were chosen as sepsis group. 35 neonates with non-infection diseases were selected as non-infection group and 40 healthy newborn infants were assigned as control group. The level of CD64 in blood were detected by flow cytometry, while the level of MCP-1, IL-8 and IP-10 of the serum in the three groups were detected by automatic biochemical analyzer. The differences between groups were compared by single factor ANOVA. The ROC curves of sepsis diagnosed by whole blood CD64, serum MCP-1, IL-8 and IP-10 were drew. Result The level of CD64, IL-8 and IP-10 of the neonatal blood in the sepsis group were significantly higher than that in the non-infection group (P 0.05), but significantly higher than that in the control group (P 0.05). The optimal thresholds of blood CD64, MCP-1, IL-8 and IP-10 in the diagnosis of sepsis were 35.0 MFI, 58.6 ng/L, 60.3 ng/L, 0.46 μg/L. The sensitivity and specificity of the diagnosis of sepsis were 92.8% and 90.6% in CD64, 70.0% and 42.6% in MCP-1, 78.5% and 68.0% in IL-8, 72.8% and 54.6% in IP-10, 97.1% and 94.6% when combined. Conclusion The combination test of CD64, MCP-1, IL-8 and IP-10 can improve the sensitivity and specificity of the diagnosis of sepsis. Key words: Sepsis; Interleukin-8; Interferon-gamma; Chemokines; CD64