Abstract
BackgroundIndividualized therapeutic regimen is a recently intensively pursued approach for targeting diseases, in which the search for biomarkers was considered the first and most important. Thus, the goal of this study was to investigate whether the UGT1A1, ERCC1, BRCA1, TYMS, RRM1, TUBB3, STMN1 and TOP2A genes are underlying biomarkers for gastric cancer, which, to our knowledge, has not been performed.MethodsNinety-eight tissue specimens were collected from gastric cancer patients between May 2012 and March 2015. A multiplex branched DNA liquidchip technology was used for measuring the mRNA expressions of ERCC1, BRCA1, TYMS, RRM1, TUBB3, STMN1 and TOP2A. Direct sequencing was performed for determination of UGT1A1 polymorphisms. Furthermore, correlations between gene expressions, polymorphisms and clinicopathological characteristics were investigated.ResultsThe expressions of TYMS, TUBB3 and STMN1 were significantly associated with the clinicopathological characteristics of age, gender and family history of gastric cancer, but not with differentiation, growth patterns, metastasis and TNM staging in patients with gastric cancer. No clinical characteristics were correlated with the expressions of ERCC1, BRCA1, RRM1 and TOP2A. Additionally, patients carrying G allele at −211 of UGT1A1 were predisposed to developing tubular adenocarcinoma, while individuals carrying 6TAA or G allele respectively at *28 or −3156 of UGT1A1 tended to have a local invasion.ConclusionsThe UGT1A1 polymorphism may be useful to screen the risk population of gastric cancer, while TYMS, TUBB3 and STMN1 may be potential biomarkers for prognosis and chemotherapy guidance.
Highlights
Individualized therapeutic regimen is a recently intensively pursued approach for targeting diseases, in which the search for biomarkers was considered the first and most important
Several studies have suggested that the expressions of excision repair cross complementing 1 (ERCC1), breast cancer susceptibility gene breast cancer 1 (BRCA1), thymidylate synthetase (TYMS), ribonucleotide reductase M1 (RRM1), β-tubulin III (TUBB3), stathmin1 (STMN1) and topoisomerase IIα (TOP2A) genes
The present study aims to detect the UGT1A1 polymorphisms and expressions of ERCC1, BRCA1, TYMS, RRM1, TUBB3, STMN1 and Topoisomerase IIα (TOP2A) in the tumor tissues from gastric cancer patients and explore their relationships with the clinicopathological characteristics, hoping to provide guidance for targeted cancer therapies individually
Summary
Individualized therapeutic regimen is a recently intensively pursued approach for targeting diseases, in which the search for biomarkers was considered the first and most important. The goal of this study was to investigate whether the UGT1A1, ERCC1, BRCA1, TYMS, RRM1, TUBB3, STMN1 and TOP2A genes are underlying biomarkers for gastric cancer, which, to our knowledge, has not been performed. Several studies have suggested that the expressions of excision repair cross complementing 1 (ERCC1), breast cancer susceptibility gene breast cancer 1 (BRCA1), thymidylate synthetase (TYMS), ribonucleotide reductase M1 (RRM1), β-tubulin III (TUBB3), stathmin (STMN1) and topoisomerase IIα (TOP2A) genes. Cao et al BMC Gastroenterology (2017) 17:2 are differential in cancer tissues and are closely associated with the clinicopathological characteristics of patients (such as clinical staging or lymphatic metastasis), which make them suitable to act as possible biomarkers to evaluate the therapeutic response and survival rates of cancer patients [3,4,5,6,7,8]. Mounting evidence indicates their important roles in gastric cancer [9,10,11,12,13,14,15], no study, to our knowledge, has been conducted to simultaneously detect their expressions in the samples of gastric cancer like other cancers [3,4,5,6,7,8]
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