Clinical significance of serum miR-187 and miR-143 in the diagnosis of gallbladder cancer

Abstract

Objective To investigate the clinical significance of serum miR-187 and miR-143 in the development and diagnosis of gallbladder cancer. Methods 75 serum samples in patients with gallbladder cancer were selected as gallbladder cancer group. 75 serum samples in patients with gallbladder benign disease and 45 serum samples in healthy physical examinations at same period were selected as the benign gallbladder disease group and healthy control group. Quantitative RT-PCR was used to detect the serum miR-187 and miR-143 expression in each group, and the expression of those related with the clinicopathological factors, the proliferation and migration of gallbladder cancer cells, and the efficacy in diagnosis of gallbladder cancer was observed. Results The serum miR-187 expression in gallbladder cancer group was significantly higher than that in benign gallbladder disease and healthy control; the serum expression of that in benign gallbladder disease was significantly higher than that in healthy control; after surgery , the expression of that was significantly lower than that before treatment (all P 0.05), and were significantly correlated with Nevin stage, TNM stage, differentiation and lymphatic metastasis (all P<0.05). Furthermore, it was confirmed that miR-187 promoted the proliferation and migration of gallbladder cancer cells in vitro, while miR-143 inhibited the proliferation and migration. In the diagnosis of gallbladder, the diagnostic efficacy of miR-187 and miR-143 was significantly better than that of CA199 and CA242 (both P<0.05). Combined detection could further improve the efficacy in diagnosis of gallbladder cancer. Conclusions miR-187 and miR-143 are involved in the development of gallbladder cancer. Combined detection of serum miR-187 and miR-143 in gallbladder cancer has a high diagnostic efficiency in the diagnosis of gallbladder cancer. Key words: Gall bladder neoplasm; MiR-187; MiR-143; Diagnosis; Real-time -PCR