Clinical significance of serum levels of microRNA-27a and its correlation with interleukin-10 in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning.

Huizhen Dai,Hongyan Wang,Xinxia Zu,Lili Wang,Huan Zhang

doi:10.3892/etm.2020.8409

Huizhen Dai, Hongyan Wang + Show 3 more

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https://doi.org/10.3892/etm.2020.8409

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Abstract

The aims of the present study were to examine the clinical significance of serum microRNA-27a (miR-27a) levels in patients with multiple organ dysfunction syndrome (MODS) caused by acute paraquat poisoning and to investigate the correlation between miR-27a and interleukin (IL)-10. A total of 82 patients with MODS induced by acute paraquat poisoning and 88 healthy controls were recruited in the present study. Reverse transcription-quantitative PCR was used to measure serum miR-27a expression levels in patients with MODS and the control group. IL-10 serum levels were determined using ELISA. Decreased serum miR-27a level and increased IL-10 expression levels were detected in patients with paraquat poisoning compared with healthy controls (P<0.001). A moderately negative correlation was identified between the serum expression levels of miR-27a and IL-10 (r=-0.5225; P<0.001). miR-27a expression level was found to be associated with blood urea nitrogen, partial pressure of carbon dioxide, arterial blood lactic acid, and the acute physiology and chronic health evaluation II score (APACHE II; P<0.05). The area under the curve for miR-27a was 0.946, with a sensitivity of 86.6% and specificity of 87.5% at a cutoff value of 2.10. The non-survival patient group had lower miR-27a expression levels compared with the survival group (P<0.01). Multivariate Cox regression analyses suggested miR-27a expression level and APACHE II score were independent prognostic factors for 30-day mortality (P<0.01). The present results suggested that serum miR-27a level may be a potential novel diagnostic and prognostic factor for MODS caused by paraquat poisoning. Collectively, miR-27a may be involved in the process of MODS induced by paraquat poisoning by regulating the inflammatory response.

Highlights

Paraquat is a type of bipyridine compound that is widely used as a herbicide [1]
A recent study reported that miR‐146a can regulate the occurrence and immune response of lung injury induced by paraquat poisoning, and that interleukin (IL)‐6 is involved in the regulation of this process [11]
The present results suggested that the serum miR‐27a expression level was significantly decreased in patients with Multiple organ dysfunction syndrome (MODS) compared with the healthy controls (Fig. 2; P

Summary

Introduction

Paraquat is a type of bipyridine compound that is widely used as a herbicide [1]. Self‐poisoning with pesticides is a major public health problem in developing countries [2]. As a result of its widespread use, paraquat is regarded as the main herbicide involved in accidental and intentional poisoning [3]. Multiple organ dysfunction syndrome (MODS) is the main cause of mortality induced by paraquat poisoning [6]. The dysregulation of the inflammatory response plays a crucial role in the development of MODS [8,9]. A number of genes that are differentially expressed during the occurrence and development of inflammation are candidate targets for gene therapy, including some microRNAs (miRNAs/miRs) [10]. A recent study reported that miR‐146a can regulate the occurrence and immune response of lung injury induced by paraquat poisoning, and that interleukin (IL)‐6 is involved in the regulation of this process [11]

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