Abstract
To investigate the relationship between plasma EBV-DNA concentration and clinicopathologic features of Hodgkin's lymphoma cases. At first, the positive rate of plasma EBV-DNA was determined with a nested-PCR method using 45 specimens from Uygur HL patients, as well as 110 healthy people sampled as normal controls. Secondly, using fluorescent quantitative nested-PCR, EBV viral load was assessed in the EBV-DNA positive plasma samples. Then, relationships between plasma EBV viral load and clinicopathologic features of HL patients were analyzed. The positive rate of plasma EBV-DNA of HL patients was significantly higher than that of normal controls (53.3%vs26.4%, P=0.001). There was no significant difference about plasma EBV viral load between EBV-associated HL and EBV-DNA positive normal people (P=0.490). Looking at patients' characteristics, plasma EBV viral load in 10-20 years EBV-associated HL was higher than in EBV cases which were less than 10 years or more than 35 years (P=0.025). Furthermore, in EBV-associated HL, concentration of plasma EBV-DNA was significantly higher in advanced stage disease (stages III-IV; P=0.013), and with B-symptoms (P=0.020). EBV-DNA levels were associated with part of clinicopathologic features of cases. It was of practical use to screen HL. Further etiological studies appear warranted.
Highlights
Epstein-Barr virus (EBV) might be one of the pathogenic factors of Hodgkin’s lymphoma (HL) and its expression rate has obvious geographical and ethnic differences (Massini et al, 2009)
At first, the positive rate of plasma EBV-DNA was determined with a nested-PCR method using 45 specimens from Uygur HL patients, as well as 110 healthy people sampled as normal controls
Paraffin tissue samples of Uygur patients with HL in Xinjiang were detected for EBV detection with in situ hybridization and it was found that positive rate was 65%
Summary
Epstein-Barr virus (EBV) might be one of the pathogenic factors of Hodgkin’s lymphoma (HL) and its expression rate has obvious geographical and ethnic differences (Massini et al, 2009). Paraffin tissue samples of Uygur patients with HL in Xinjiang were detected for EBV detection with in situ hybridization and it was found that positive rate was 65%. This region could be regarded as an area with geographic advantages of HL incidence research. That provided part of the clue for discussion of pathogenic factors of HL in this region It laid the foundation for the deep research on the clinical value of plasma EBV-DNA quantitative analysis in HL early diagnosis, prognosis and monitoring of recurrence and metastasis after treatment in the future
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