Abstract
The frequency of p53 and Bcl-2 protein expression in 100 newly diagnosed and 10 relapsed acute myeloid leukemia (AML) patients was analyzed by immunocytochemistry (ICC). The Kaplan-Meier method was used for univariate and multivariate statistical analysis to assess the relationship between p53, Bcl-2 and clinico-hematologic feature with respect to overall survival (OS) using SPSS statistical software. No statistical significance was found in univariate analysis (P=0.60). However, when the subgroups of patients (+1, +2, +3 and +4) were compared, expression of p53 and Bcl-2 protein (1–10%, 11–30%, 31–50% and >50%) was statistically significant (P<0.05). However, in multivariate analysis, p53, immunopositivity was independently associated with a shorter overall survival (OS) (P=0.038) while Bcl-2 immunopositivity was associated with longer overall survival (OS) (P=0.002). Our finding shows that p53 and Bcl-2 protein overexpression is a strong indicator of response to chemotherapy and overall survival. This study reports for the first time AML in patients from Eastern India.
Highlights
The patients with acute myeloid leukemia but gm/m2) in a 15 mL centrifuge tube; the propor- slides were rinsed in TBS and counterwhen present is associated with a low com- tion between the Ficoll Hypaque layer and stained with hematoxylin
Ship between p53 and Bcl-2 protein expression ly Results on Immunostaining for p53 and Bcl-2 was successful in 110 acute myeloid leukemia (AML) patients
Discussion p53 and Bcl-2 immunostaining data of AML patients were evaluated to establish a possible correlation with overall survival outcome (Tables 1 and 2)
Summary
This study includes 100 de novo and 10 relapsed de novo AML patients diagnosed in Attribution NonCommercial 3.0 License (CC BYNC 3.0). These slides were incubated for 10 min in 1.5% hydrogen peroxide. After washing in TBS, a secondary biotinylated goat anti-mouse/rabbit antibody (Dako Corporation, Denmark) was applied for 30 and cannot be detected by immunocytochem- Using heparin as an anti-coagulant, 3 ml of min. The patients with acute myeloid leukemia but gm/m2) in a 15 mL centrifuge tube; the propor- slides were rinsed in TBS and counterwhen present is associated with a low com- tion between the Ficoll Hypaque layer and stained with hematoxylin. Slides were scored as +1 (1-10%), +2 (11-30%), +3 (31-50%) and +4 (>50% positive cells).[25,24]
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