Clinical significance of myeliod-derived suppressor cells in peripheral blood and serum interleukin-10 level detection in children with bronchiolitis

Abstract

Objective To explore the clinical significance of detecting serum interleukin-10(IL-10) level and the proportion of myeloid-derived suppressor cells (MDSCs) in peripheral blood mononuclear cells(PBMCs) in children with bronchiolitis. Methods Fifty-one children with bronchiolitis less than 2 years old were randomly enrolled including 27 boys and 24 girls.They were divided into 2 groups: bronchiolitis group Ⅰ, 25 children with atopic high risks were included in this group; bronchiolitis group Ⅱ, 26 children without atopic high risks were included in this group.Children without infectious diseases such as hernia and renal calculus had been randomly enrolled as the control group(without atopic disease and atopic family disease) (n=45), including 25 boys and 20 girls.After taking 4 mL venous blood of patients in 3 groups, 1 mL was used to test the serum proportion of MDSCs by flow cytometry, and the remaining blood was used to test the level of IL-10 in the serum by enzyme-linked immunosorbent assay. Results 1.The proportions of MDSCs in the PBMCs in bronchiolitis groupⅠ[(3.17±0.24)%] and bronchiolitis groupⅡ[(1.33±0.25)%] were significantly higher than that of control group [(0.78±0.25)%](all P 0.05). Conclusion MDSCs plays a crucial role by up-regulating the IL-10 level in the process of developing asthma from bronchiolitis. Key words: Bronchiolitis; Interleukin-10; Myeliod-derived suppressor cell; Infant