Abstract
miRNAs play an indispensable role in human carcinogenesis. Dysregulated miR-1180-3p has been observed in several types of cancer, including hepatocellular carcinoma (HCC). This study intends to correlate the expression level of miR-1180-3p with clinical features and overall survival in HCC patients. The expression and clinical significance of miR-1180-3p, selected from GEO and TCGA databases, were verified using an RT-qPCR method. The target genes of miR-1180-3p were obtained using 3 miRNA target gene prediction databases, and their functions were analyzed using the online tool WebGestalt. miR-1180-3p expression was significantly upregulated in 88 HCC tissues compared with non-tumor liver tissues (0.004 ± 0.009 vs. 0.002 ± 0.002, t = − 2.099, P = 0.038). Additionally, we found that the expression levels of miR-1180-3p were significantly correlated with tumor number (χ2 = 9.157, P = 0.006) and MVI (χ2 = 11.354, P = 0.003). Based on Kaplan–Meier analysis, patients with high miR-1180 expression had a shorter overall survival than those with low miR-1180-3p expression (P = 0.002). Furthermore, multivariate Cox analyses indicated that miR-1180-3p expression was an independent prognostic factor for overall survival (HR = 13.36, 95% CI 1.16, 153.69, P = 0.038). In addition, a total of 733 target genes of miR-1180-3p were found from three prediction databases. The GO analyses demonstrated that the target genes were closely related to the proliferation and malignancy of tumors. The KEGG analysis showed that target genes were enriched in several key cancer-related signaling pathways, including the Pathways in cancer, the Ras signaling pathway, and the MAPK signaling pathway. In conclusion, we demonstrate that miR-1180-3p is upregulated in HCC and is associated with a poor prognosis. Thus, miR-1180-3p might be useful as a prognostic marker for HCC.
Highlights
MiRNAs play an indispensable role in human carcinogenesis
We further analyzed the relationship between three upregulation miRNA and the prognosis of hepatocellular carcinoma (HCC) using the TCGA database, and found high expression levels of miR-1180-3p were correlated closely with poor outcomes of HCC patients
We aimed to screen the abnormally expressed miRNAs in HCC. 14 significantly differentially expressed miRNAs (|Log2FC|≥ 1.2 and false discovery rate (FDR) < 0.001), including 3 upregulated miRNAs and 11 downregulated miRNAs, were identified in the GSE36915 dataset (Fig. 1). These results indicated that miR-183-5p (log2FC = 2.36, P < 0.001), miR452-3p (log2FC = 1.73, P < 0.001), and miR-1180-3p (log2FC = 1.21, P < 0.001) may act as oncomiRs in HCC
Summary
MiRNAs play an indispensable role in human carcinogenesis. Dysregulated miR-1180-3p has been observed in several types of cancer, including hepatocellular carcinoma (HCC). This study intends to correlate the expression level of miR-1180-3p with clinical features and overall survival in HCC patients. MiR-1180-3p expression was significantly upregulated in 88 HCC tissues compared with non-tumor liver tissues (0.004 ± 0.009 vs 0.002 ± 0.002, t = − 2.099, P = 0.038). MiR-1180-3p is expressed from chromosome 17 (p11.2), and this miRNA may play different roles in carcinogenesis as drivers, oncogenes and tumor s uppressors[18,19,20]. We further analyzed the relationship between three upregulation miRNA and the prognosis of HCC using the TCGA database, and found high expression levels of miR-1180-3p were correlated closely with poor outcomes of HCC patients. We validated miR-1180-3p expression using 88 pairs of HCC tissues and matched non-tumor liver tissues and investigated the clinical significance of miR-1180-3p in HCC. To thoroughly understand the biological function and pathways of miR-1180-3p, we predicted the miR-1180-3p target genes, followed by functional enrichment analysis
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