Abstract
To investigate the changes of serum vascular endothelial growth factor (VEGF), soluble interleukin-2 receptor (SIL-2R) and hepatocyte growth factor (HGF) contents in patients with primary hepatocellular carcinoma (HCC) before and after percutaneous microwave coagulation therapy (PMCT) and determine their clinical significance. Fasting venous blood (3 mL) from 81 patients with primary HCC diagnosed by pathology was collected in the mornings 1 day before PMCT, and 1 day, 7 days and 1 month after PMCT, and then the serum was separated and stored in -70°. The contents of VEGF, SIL-2R and HGF were detected by enzyme linked immunosorbent assay (ELISA). The serum VEGF, SIL-2R and HGF contents in 81 patients with primary HCC had obviously dynamic changes before and after PMCT. By comparison to 1 day after PMCT with pre-operation, there was no statistical significance regarding VEGF and SIL-2R contents (P>0.05), but HGF content showed significant difference (P<0.01). Compared with pre-operation, VEGF, SIL-2R and HGF contents 7 days and 1 month after PMCT all manifested significant differences (P<0.01). By comparison to 7 days with 1 month after PMCT, there was no statistical significance regarding the VEGF content (P>0.05), whereas SIL-2R and HGF contents showed significant change (P<0.01). The contents of serum VEGF, SIL-2R and HGF have obviously dynamic changes in primary HCC before and after PMCT, and their joint detection is expected to be an effective hematologic evaluation index of PMCT for primary HCC.
Highlights
Degree of tumor necrosis The ultrasound imaging or enhanced MRI was conducted in 81 patients 1 month after microwave treatment, and the results showed that all of them had no residual carcinoma and recurrence of liver inside and outside
VEGF is a currently-known tumor-related angiogenesis factor with the strongest activity for promoting the proliferation and division of vascular endothelial cells in the body (Ahluwalia et al, 2012). It is highly expressed in liver cancer tissue together with vascular endothelial growth factor receptor (VEGFR)
After percutaneous microwave coagulation therapy (PMCT), a large number of liver cancer cells were killed, which can affect the change of VEGF content by decreasing VEGF secretion (Cui et al, 2005)
Summary
Primary hepatocellular carcinoma (HCC) is one of the most commonly-encountered malignant tumors in the world (Abdelgawad et al, 2013; Berk et al, 2013; Chittmittrapap et al, 2013; Gupta et al, 2013; Norsa’adah et al, 2013; Hao et al, 2013; Zekri et al, 2013; Zhu et al, 2013; Cai et al, 2014; El Kassas et al, 2014; Fan et al, 2014; Ji et al, 2014; Jeng et al, 2014; Li et al, 2014; Tang et al, 2014; Wang et al, 2014; Zhang et al, 2014). Modern imaging examinations have been able to reflect the real situation of tumor tissue necrosis timely, but it still needs to find its hematologic monitoring index (Zhan et al, 2013). We are hoping to find a good repeatable, convenient and effective hematologic evaluation index that can reflect the efficacy of PMCT timely. Vascular endothelial growth factor (VEGF) is the most important angiogenesis factor in tumor tissues (Pircher et al, 2014). Serum soluble interleukin-2 receptor (SIL–2R) is an important monitoring index which can reflect the body cellular immunity function (Kanazawa et al, 2006). Hu et al found that serum HGF concentration was increased significantly on day 1 after hepatectomy, suggesting that the process of early liver regeneration was launched after microwave treatment (Hu et al, 1999). This study detected the content changes of serum VEGF, SIL-2R and HGF in patients with primary HCC before and after PMCT by enzyme linked immunosorbent assay (ELISA) in order to provide some hematologic evidences for judging the efficacy of PMCT for primary HCC and prognosis
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