Clinical significance of human telomerase RNA component and C-myc gene expression in cervical cancer and precancerous lesions

Abstract

Objective To investigate the expression level of human telomerase RNA component (hTERC) and C-myc in cervical lesions.Methods Fluorescence in situ hybridization (FISH) was applied to detect hTERC and C-myc expression in 62 cases of cervical tissue including 35 cases of cervical intraepithelial neoplasia,8 cases of invasive cervical cancer,19 cases of inflammation as controls.Results The positive rates of hTERC on chromosome 3 in chronic cervicitis organizations,CIN Ⅰ,CIN Ⅱ-Ⅲ and cervical cancer were 5.3 ％ (1/19),16.7 ％(2/12),87.0 ％ (20/23),87.5 ％ (7/8) (x2 =36.299,x2 =40.237,P ＜0.01).The positive rates of C-myc in chronic cervical inflammation organization,CIN Ⅰ,CIN Ⅱ-Ⅲ and cervical cancer were 5.3 ％ (1/19),8.3 ％ (1/12),78.3 ％ (18/23),62.5 ％ (5/8) (x2 =30.200,x2 =34.224,P ＜0.01 ).The differences among groups were statistically significant.hTERC had a positively correlation with C-myc expression (r =0.514,P ＜ 0.01).Conclusion The expression of hTERC and C-myc on chromosome 3 is closely related to cervical cancer progression. Key words: Uterine cervical neoplasms; Human telomerase RNA component; Genes, myc