Abstract
BackgroundmicroRNA-381 is dysregulated in a variety of cancers. However, its clinical significance in pediatric acute myeloid leukemia (AML) is still unclear. The purpose of this study was to detect the expression level of miR-381 in pediatric AML patients and to explore its potential clinical significance.MethodsThe levels of miR-381 in bone marrow and serum of 102 pediatric AML patients were measured by quantitative real-time polymorperase chain reaction (qRT-PCR). The diagnostic value of serum miR-381 in pediatric AML patients was evaluated by the receiver operating characteristic (ROC) curve. A Chi square test was used to analyze the relationship between serum miR-381 and the clinical characteristics of patients. Cox regression analysis and Kaplan–Meier evaluated the prognostic value of serum miR-381 in patients. Finally, the proliferation of the cells was analyzed by the CCK-8 assay.ResultsCompared with healthy controls, the levels of miR-381 in serum and bone marrow of pediatric AML patients were significantly decreased (P < 0.001). ROC curve showed that miR-381 could distinguish pediatric AML cases from normal controls. At the same time, the downregulation of miR-381 was associated with M7 in the French–American–British (FAB) classifications and unfavorable cytogenetic risks (P < 0.05). Low serum miR-381 levels were associated with poor overall survival of pediatric AML (log-rank test, P = 0.011) and poor relapse-free survival (log-rank test, P = 0.004). Cox regression analysis confirmed that reduced serum miR-381 was an independent predictor of poor prognosis in AML (HR = 3.794, 95% CI 1.3633–10.559, P = 0.011). In addition, low expression of miR-381 significantly reduced the proliferation of cells (P < 0.05).ConclusionAll experimental results confirm that miR-381 has reduced bone marrow and serum expression in pediatric AML, and low levels of serum miR-381 have certain diagnostic and prognostic value in pediatric AML and may be a potential therapeutic target for AML.
Highlights
MicroRNA-381 is dysregulated in a variety of cancers
Decreased expression of miR‐381 in patients with pediatric acute myeloid leukemia (AML) To investigate the level of mIR-381 expression in pediatric AML, quantitative real-time polymorperase chain reaction (qRT-PCR) was used to analyze bone marrow and serum from 102 patients and 50 healthy control
It was noted that the expression of miR-381 in the bone marrow and serum was significantly positively correlated (r = 0.7149, P < 0.001, Fig. 1c)
Summary
Its clinical significance in pediatric acute myeloid leukemia (AML) is still unclear. The purpose of this study was to detect the expression level of miR-381 in pediatric AML patients and to explore its potential clinical significance. MicroRNAs (miRNAs) are non-coding RNA molecules composed of 19–22 nucleotides that can play a critical role as oncogenes or tumor suppressor genes in the development and progression of multiple solid or blood tumors, including AML. MiR-10a/b is abnormally highly expressed in AML patients and promotes the proliferation of promyelocytic leukemia [7]. Due to its high conservatism, stability, sensitivity, and extensive presence in tissues and body fluids (blood, saliva, plasma, and serum), miRNAs have been widely studied as biomarkers for clinical diagnosis and prognosis of a variety of diseases
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