Clinical significance of combined detection of human papillomavirus L1 capsid protein,thinprep cytologic test and human papillomavirus genotyping on the early diagnosis of cervical cancer

Abstract

Objective To investigate the clinical significance of combined detection of human papillomavirus (HPV) L1 capsid protein,thinprep cytoligic test (TCT) and HPV genotyping on the early diagnosis of cervical cancer.Methods A total of 1 260 patients with sexual life history and cervical lesions were detected of HPV genotyping and TCT,then one or both in the two indicators was tested by biopsy.Meanwhile the expression of HPV L1 capsid protein was detected in patients with abnormal TCT.Contrasted TCT,HPV genotyping,HPV L1 capsid protein and histopathological diagnosis.Results The positive rate of cervical intraepithelial neoplasia (CIN) and more gradually of no definitive diagnosis significance of atypical squamous cells (ASCUS),low-grade squamous epithelial cell lesions (LSIL),the height of squamousintraepithelial lesion (HSIL),squamous cell carcinoma (SCC) was respectively 19.0％ (11/58),63.4％ (26/41),84.6％ (22/26) and 7/7,which increased with TCT-level rise,and there was significant difference (P ＜ 0.01).Low risk HPV-positive were mainly seen in low-grade lesions,with the TCT-level rise,high risk HPV-positive rate was significantly higher,which was respectively 36.2％ (21/58),56.1％ (23/41),73.1％(19/26) and 7/7,and there was significant difference (P＜0.05).The rate ofinflammation,CIN Ⅰ in HPV L1 capsid protein-positive was higher than HPV L1 capsid protein-negative [59.2％ (42/71) vs.39.3％ (24/61),29.6％(21/71) vs.14.8％(9/61)],pathological diagnosis of SCC were negative in HPV L1 capsid protein expression.With the increased level of pathology,the positive rate of HPV L1 capsid protein decreased.Conclusions That TCT liquid-based cytology and HPV genotyping can significantly reduce high-risk groups and significantly improve the detection rate of cervical cancer,and to detect HPV L1 capsid protein of HPV infection may provide the basis for the status and prognosis.Combination of the three indicators can provide the basis for early diagnosis of cervical cancer. Key words: Human papillomavirus; Uterine cervical neoplasms; Cervical intraepithelial neoplasia