Abstract
BackgroundThe aim of the study was to test for human papilloma virus (HPV) infection and human telomerase RNA component (hTERC) gene amplification in tissues derived from esophageal cancer, in esophagus displaying atypical hyperplasia and in normal tissue, and to analyze the relationship between them and discuss whether HPV infection and hTERC gene amplification play a role in the duration of survival of esophageal cancer patients.MethodsTo test for HPV infection, surface plasma resonance was used after extracting and subjecting the DNA to PCR amplification. Measurement of hTERC gene amplification was performed by the fluorescence in situ hybridization technique.ResultsThe rates of HPV infection in the normal group, the atypical esophageal hyperplasia group and the cancer group were 0% (0/40), 10.00% (1/10) and 20.65% (19/92), respectively, with a statistically significant difference of P < 0.01. The hTERC gene amplification rate in normal tissue, grade I atypical hyperplastic tissue, grade II/III atypical hyperplastic tissue and esophageal cancer tissue were 0% (0/89), 15.38% (4/26), 47.06% (8/17) and 89.13% (82/92), respectively, with a statistically significant difference of P < 0.01. On follow-up of 92 patients, survival curves of the HPV-positive and HPV-negative groups were not significantly different (P > 0.05). Survival curves of the hTERC gene amplification-positive and hTERC gene amplification-negative groups were statistically significant (P < 0.05). A matching chi-square test showed that there was no correlation between HPV infection and hTERC gene amplification (P > 0.05).ConclusionHPV infection may be one of many factors contributing to the development of esophageal cancer, but it does not influence prognosis. Amplification of the hTERC gene appears to influence certain features associated with postoperative survival in esophageal carcinoma patients.
Highlights
The aim of the study was to test for human papilloma virus (HPV) infection and human telomerase RNA component gene amplification in tissues derived from esophageal cancer, in esophagus displaying atypical hyperplasia and in normal tissue, and to analyze the relationship between them and discuss whether HPV infection and hTERC gene amplification play a role in the duration of survival of esophageal cancer patients
Increasing epidemiologic and biologic evidence indicates that human papilloma virus (HPV) infection is associated with the occurrence of Squamous cell carcinoma of the esophagus (ESCC) [1,2,3]
By taking into account that ESCC and cervical cancer are both squamous cell carcinomas, we explored whether HPV infection and the hTERC gene in ESCC display similar mechanisms in the pathogenesis of cervical cancer
Summary
The aim of the study was to test for human papilloma virus (HPV) infection and human telomerase RNA component (hTERC) gene amplification in tissues derived from esophageal cancer, in esophagus displaying atypical hyperplasia and in normal tissue, and to analyze the relationship between them and discuss whether HPV infection and hTERC gene amplification play a role in the duration of survival of esophageal cancer patients. The prognosis of ESCC is very poor because of the paucity of symptoms seen in these patients at the earliest stages of disease. The human telomerase RNA component (hTERC) gene may influence the integration of HPV into the cellular genome, accumulation of numerical chromosome aberrations and development of genomic instability with a consistent gain of chromosome arm 3q. Hopman and colleagues showed that both the genomic integration of oncogenic HPV and gain of the hTERC gene appeared to be important genetic events that were associated with the progression of uterine cervical dysplasia to an invasive cancer [4]. By taking into account that ESCC and cervical cancer are both squamous cell carcinomas, we explored whether HPV infection and the hTERC gene in ESCC display similar mechanisms in the pathogenesis of cervical cancer
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