Clinical Significance of Circulating Serum Levels of sCD95 and TNF-α in Cytoprotection of Cervical Cancer.

Mahendra Pal Singh Negi,Madan Lal Brahma Bhatt,Raj Kamal Tripathi,Saurabh Kumar Agnihotri,Ankita Jain,Rekha Sachan,Balawant Kumar,Anjali Anjali,M S Sachdev

doi:10.52547/rbmb.10.4.711

Abstract

This study correlates the serum levels of sCD95 & TNF-α with a simple cell-based assay to evaluate the capacity of the serum sample to induce apoptosis in Jurkat cells. Interlinking of these parameters can be explored to design a minimum invasive diagnostic strategy for cervical cancer (CC). Sera samples were assessed to induce apoptosis in Jurkat cells through FACS. Serum levels of sCD95 and TNF-α were measured by ELISA. JNK phosphorylation was evaluated in sera incubated Jurkat cells. Data was scrutinized through statistical analysis. Significantly higher serum levels of sCD95 and lower TNF-α levels were observed in CC patients; their sera samples inhibited induction of apoptosis in Jurkat cells through reduced JNK phosphorylation. Statistical analysis linked these three parameters for the early screening of CC. Distinct sera levels of sCD95 & TNF-α in CC patients showed an anti-apoptotic effect, which can be considered for early detection of CC.

Highlights

Apoptotic pathways are usually disrupted or inactivated in cancerous cells and engenders uncontrolled cell growth and tumor cell resistance [1]
Further assessment for the p38 phosphorylation in the similar experimental set up demonstrated higher phosphorylation with the CC patients’ sera, when compared with sera of healthy women. These results strongly suggest that apoptosis induction in Jurkat T cell is mediated by CD95/CD95L interactions that activate the JNK1 and p38 downstream
It is known that CD95-ligand binds to the sCD95, which further protect the cancerous cells from CD95-mediated apoptosis and represents one of the method of evading immune surveillance [2, 28]

Summary

Introduction

Apoptotic pathways are usually disrupted or inactivated in cancerous cells and engenders uncontrolled cell growth and tumor cell resistance [1]. This study correlates the serum levels of sCD95 & TNF-α with a simple cell-based assay to evaluate the capacity of the serum sample to induce apoptosis in Jurkat cells Interlinking of these parameters can be explored to design a minimum invasive diagnostic strategy for cervical cancer (CC). Results: Significantly higher serum levels of sCD95 and lower TNF-α levels were observed in CC patients; their sera samples inhibited induction of apoptosis in Jurkat cells through reduced JNK phosphorylation. Statistical analysis linked these three parameters for the early screening of CC. Conclusions: Distinct sera levels of sCD95 & TNF-α in CC patients showed an anti-apoptotic effect, which can be considered for early detection of CC

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Conclusion