Clinical Significance, Cellular Function, and Potential Molecular Pathways of CCT7 in Endometrial Cancer.

Hui Yang,Liwen Wang,Nianchun Shan,Wei Zhou,Hui Li

doi:10.3389/fonc.2020.01468

Hui Yang, Liwen Wang + Show 3 more

Open Access

https://doi.org/10.3389/fonc.2020.01468

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Abstract

Objective: Endometrial cancer (EC) is a common gynecologic malignancy; myometrial invasion (MI) is a typical approach of EC spreads and an important index to assess tumor metastasis and outcome in EC patients. CCT7 is a member of the TCP1 chaperone family, involved in cytoskeletal protein folding and unfolding. In this study, the role of CCT7 in EC development was investigated.Methods: Clinical data for 87 EC cases and expression of CCT7 were analyzed. CCT7 was knocked out using siRNA-CCT7 in Ishikawa and RL95-2 cells, and their function about proliferation, apoptosis, and invasion was further tested. Bioinformatics methods were used to predict the potential pathways of CCT7 in EC development.Results: The rates of CCT7-positive cells in EC and adjacent normal endometrium tissues had a significant difference (67.8 vs. 51.4%, p = 0.035), and the expression rate increased from low to high pathological stage (39.7% in the I/II stage, 71.4% in the III/IV stage, p = 0.029). A similar change was found in protein level. CCT7 expression differed significantly between the deep MI group (>1/2) and the superficial MI group (≤1/2) (p = 0.039). However, there were no differences with respect to age, pathological type, and histological grade. CCT7 suppression induced a function loss in both Ishikawa and RL95-2 cells. Bioinformatics analysis demonstrated that EC patients with lower-level CCT7 expression had better overall survival (p = 0.0081). Gene ontology enrichment indicated that “RNA binding,” “Mitochondrion,” “Translation,” and “Spliceosome” were most significantly enriched potential pathways. Five hub genes, PSMA5, PSMD14, SNRPB, SNRPG, and TXNL4A, were all significantly upregulated in EC and had a positive correlation with CCT7.Conclusions: CCT7 may be involved in EC development by excessively activating tumor cell function to promote MI or distant/nodal metastasis, which may contribute to the prognosis of EC patients.

Highlights

MATERIALS AND METHODSEndometrial cancer (EC) is the fourth highest malignant tumor Tissue Sample Collection in women and the most common gynecologic cancer of the This retrospective study was approved by the Ethics Committees female reproductive system in the United States, with an overall of Xiangya Hospital, Central South University
Bioinformatics analysis demonstrated that EC patients with lower-level CCT7 expression had better overall survival (p = 0.0081)
Shan N (2020) Clinical Significance, Cellular Function, and Potential Molecular Pathways of CCT7 in Conclusions: CCT7 may be involved in EC development by excessively activating tumor cell function to promote myometrial invasion (MI) or distant/nodal metastasis, which may contribute to the Endometrial Cancer

Summary

Objective

Endometrial cancer (EC) is a common gynecologic malignancy; myometrial invasion (MI) is a typical approach of EC spreads and an important index to assess tumor metastasis and outcome in EC patients. The role of CCT7 in EC development was investigated

Methods

Results

Conclusions

MATERIALS AND METHODS

D Cell Proliferation Assay

RESULTS

DISCUSSION

CONCLUSION

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