Clinical significance and identification analysis of differentially expressed proteins (TM9SF1 and AZU1) in the wall of ruptured intracranial aneurysms

Abstract

Objective To further identify the differentially expressed proteins (TM9SF1and AZU1) screened in our previous study in order to explore their roles in formation and rupture of intracranial aneurysms (IAs).Methods Twenty ruptured IAs underwent microsurgical clipping.During the procedure,a segment of the aneurysm dome was resected and immunostained with monoclonal antibodies for TM9SF1 and AZU1.A segment of the superficial temporal artery from the same patient was also removed and used as control group.Western blot analysis was performed to confirm the expression profile of two proteins.Results All 20 aneurysm tissues stained positive for TM9SF1 and AZU1,while negative or poor positive in STA tissues.Their expression was more abundant (P ＜ 0.05) in ruptured IA tissues than STA tissues,based on a semiquantitative grading.According to western blot analysis,relative expression of TM9SF1 and AZU1 was 4.2 and 5.6 respectively in IA tissues compared with STA tissues.Conclusions TM9SF1/AZU1 are more abundantly expressed in the wall of human ruptured IAs than in STA tissues.We speculate that the proteins may participate in formation and rupture of IAs. Key words: Intracranial aneurysm; Rupture; Immunohistochemistry; Western blot