Abstract
Objective. Discuss the changes and roles of PAF in the reproductive tract infection by observing the expression of platelet activating factor (PAF) and tumor necrosis factor α (TNF-α) in seminal plasma of patients with leukocytospermia. Methods. The seminal plasma was obtained from 22 cases of leukocytospermia and 15 cases of normal males; the peroxidase dyeing method was adopted for seminal plasma white blood count; the ELISA was adopted to test PAF and TNF-α concentration in seminal plasma. Result. PAF concentration (2.14 ± 0.43 ng/mL) of leukocytospermia group was significantly lower than the normal group (6.21 ± 1.38 ng/mL, P < 0.01) while TNF-α (5.51 ± 1.46 ng/mL) was significantly higher than that of normal group (3.48 ± 1.08 ng/mL). There was negative correlation between PAF and TNF-α , (r = −0.68, P < 0.01); the same situation existed in PAF and WBC (r = −0.62, P < 0.01); but TNF-α was positively correlated to WBC (r = 0.77, P < 0.01). Conclusion. (1) Low expression of PAF and high expression of TNF-α in leukocytospermia affect the sperm motility, which is one of the reasons that leads to infertility. (2) Lower expression of PAF has its particularity during the reproductive tract infection.
Highlights
Leukocytospermia is a kind of common disease of male reproductive tract infection, which is usually caused by bacterial infection
Leukocytospermia refers to the increase of white blood cell (WBC) density in seminal plasma which is more than 1 × 109/L, and it is mainly caused by the reproductive tract infection
The affection of these cell factors, the sperm quality, density and its vigor get lower; sperm capacitation and acrosomal reaction would be affected as well, which lead to male infertility
Summary
Leukocytospermia is a kind of common disease of male reproductive tract infection, which is usually caused by bacterial infection. An increase of white blood cells in seminal plasma can lead to the decrease of sperm quality and the increasing number of inflammatory cytokines such as interleukin IL-6 is the main factor involved in the process [1]. PAF is a kind of cytokine which has many kinds of biological activities, such as inflammation factors and sperm cell activation factors. How does the PAF change and what role does it play the role during the reproductive tract infection? The purpose of our study was to observe the expression of PAF and TNF-α in seminal plasma of patients with leukocytospermia How does the PAF change and what role does it play the role during the reproductive tract infection? The purpose of our study was to observe the expression of PAF and TNF-α in seminal plasma of patients with leukocytospermia
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