Abstract
Background The incidence and mortality of breast cancer in the world remain high. The function and important role of miR-451 and miR-506 in a series of cancers have been proved. The purpose of this research was to explore the clinical diagnosis and prognostic significance of miR-451 and miR-506 expression in breast cancer. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect miR-451 and miR-506 expression in serum and tissues. The relationship of miR-451 and miR-506 with clinical parameters was determined by the chi-square test. Receiver operating characteristics (ROC) analysis was conducted to evaluate the diagnostic accuracy of miR-451 and miR-506 in breast cancer. In addition, we determined the prognostic performance of miR-451 and miR-506 using Kaplan–Meier survival assay. Results The expression of miR-451 and miR-506 in breast cancer patients was significantly lower than that in healthy people. miR-451 and miR-506 expression decreased in breast cancer tissues compared with paracancerous tissue. High expression of miR-451 and miR-506 was associated with positive lymph node metastasis and late tumor node metastasis stage. Breast cancer patients with high miR-451 and miR-506 expression had lower five-year survival rate. The level of miR-451 and miR-506 expression showed high diagnostic accuracy for distinguishing breast cancer patients and healthy people. Conclusion miR-451 and miR-506 could be used as biomarker for the diagnosis and prognosis of breast cancer.
Highlights
Breast cancer, one of the most frequent malignant tumors throughout the world, is the second and fifth most common cancer-induced female mortality in the USA and China, respectively [1, 2]. e incidence rate of breast cancer is currently the highest in the incidence rate of cancer
Patients who underwent radiotherapy and chemotherapy before sampling were excluded. e clinical data of patients were obtained and recorded for further analysis, as given in Table 1. e staining results of estrogen receptor (ER) or progesterone receptor (PR) were considered to be positive when more than 10% cells were positive nuclear staining. e result for human epidermal growth factor receptor 2 (HER-2) staining was determined following the published UK guidelines [17]. e tumors were staged according to the 2009 Union for International Cancer Control (UICC) TNM system [18]
We found that compared with the healthy samples, miR-451 and miR-506 expression was decreased in the serum of breast cancer patients (Figures 1(a) and 1(b))
Summary
One of the most frequent malignant tumors throughout the world, is the second and fifth most common cancer-induced female mortality in the USA and China, respectively [1, 2]. e incidence rate of breast cancer is currently the highest in the incidence rate of cancer. More and more studies are focusing on identifying tumor biomarkers or therapeutic targets to enhance early diagnosis and treatment of breast cancer. Biological studies showed that miRNAs mainly affect the growth and progress of tumor cells through regulating the expression of target genes [8]. The clinical significance of miRNAs in diagnosis and prognosis has been proved in breast cancers, such as miR-940 [12], miR4317 [13], and miR-1908-3p [14]. Relationship between the expression of miR-451 and miR-506 and breast cancer diagnosis and prognosis has not yet been reported. E purpose of the present study was to investigate the expression of miR-451 and miR-506 in serum and tissue, clarify the relationship between miR-451 and miR-506 and clinical features, and further measure their clinical value of diagnosis and prognosis in breast cancer patients
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