Abstract
Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.
Highlights
Fungal species belonging to the Fusarium genus have the tremendous ability to colonize extremely different environments from soil to miscellaneous living organisms, and as far as the dining table of the international space station [1]
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Our study shed light on the species distribution of Fusarium spp. isolates originating from clinical samples and confirmed the usefulness of mass spectrometry for accurate identification of uncommon fungal pathogens
Summary
Fungal species belonging to the Fusarium genus have the tremendous ability to colonize extremely different environments from soil to miscellaneous living organisms, and as far as the dining table of the international space station [1]. They are responsible for infections in plants, animals and humans and are responsible for the degradation of organic matter including books, fabrics or paintings [2]. Fusarium spp. illnesses encompass a variety of superficial, invasive or disseminated pathologies called fusariosis. Most human invasive fusariosis is caused by Fusarium solani species complex (FSSC) and Fusarium oxysporum species complex (FOSC) but the first complex is more resistant to amphotericin B and voriconazole than the second [4]
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