Clinical Observation of Salvianolic Acid Combined with Panax Notoginseng Saponins Combined with Basic Nursing Intervention on Cerebral Ischemia-Reperfusion Injury in Rats.

Zhaopeng Zheng,Pengfei Liu,Shasha Sun,Ling Yu,Shanshan Liang,M.A Bhagyaveni

doi:10.1155/2022/8706730

Abstract

Objective To analyze the clinical observation of salvianolic acid combined with panax notoginseng saponins combined with basic nursing intervention on cerebral ischemia-reperfusion injury in rats and its effects on the expression of apoptosis-related proteins Bcl-2, Bax and caspase-3. Methods A total of 72 male Wistar rats were randomly divided into sham, ischemia/reperfusion (I/R), edaravone (Eda), salvianolic acid (SA), panax notoginseng saponins (PNS), and SA+PNS group. After administration for 5 days, the neurological function, cerebral infarction volume, brain index, and brain water content of rats were observed. ELISA kit assay was applied to measure the levels of IL-1β, IL-6, IL-8, TNF-α, MDA, SOD, GSH-Px, and T-AOC activity. Western blotting assay was used to detect the protein levels of p-53, NF-κB, Bcl-2, Bax, and Caspase-3 in the brain tissues surrounding infarction lesion. Results Compared with sham group, the mNSS score, brain index, brain water content, infarction volumes, MDA activity, and the levels of IL-6, IL-8, TNF-α and IL-1β as well as the protein levels of p-53, NF-κB, Bax and Caspase-3 were significantly increased, while the levels of Bcl-2 protein, SOD, GSH-Px and T-AOC were significantly decreased in I/R group. However, these levels were reversed in SA group, PNS group and SA + PNS group. Moreover, these changes in SA + PNS group were more obvious than those in SA and PNS group, and the differences were statistically significant. Conclusions SA, PNS and they combined with basic nursing have protective effects on cerebral I/R injury, and the combination with basic nursing has better effects than that used alone. The mechanism may be to regulate the expression of downstream apoptotic proteins by inhibiting the TLR4/NF-κB signaling pathway, thereby reducing neurological damage in rats.

Highlights

Cerebral infarction, known as cerebral ischemic stroke, is a common disease of the nervous system, which causes the obstruction or blockage of brain blood supply, resulting in oxygen and nutrient deficiency, irreversible damage to local brain tissue, leading to brain tissue ischemia and hypoxic necrosis [1]
We investigated the neuroprotective effects of salvianolic acid combined with panax notoginseng saponins on cerebral I/R injury in rats and the effects of apoptosis related proteins Bcl-2, Bax and caspase-3
salvianolic acid (SA) were purchased from Tianjin Tasly Zhijiao Pharmaceutical Co., Ltd.; panax notoginseng saponins (PNS) was purchased from Yunnan Teana Pharmaceutical Co., Ltd.; Eda was purchased from Sinopharm Group Guorui Pharmaceutical Co., Ltd.; SOD, MDA, GSH-Px and T-AOC detection kits were purchased from Shanghai Hengyuan Biotechnology Co., Ltd.; Chloral hydrate was purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.; None Hydroethanol was purchased from Chengdu Chemical Reagent Factory; Nitrogen chloride phenyltetrazolium (TTC) was purchased from Hubei Bojie Biotechnology Co., Ltd.; TNFα, IL-6, IL-8, IL-1β kits were purchased from Wuhan

Summary

Objective

To analyze the clinical observation of salvianolic acid combined with panax notoginseng saponins combined with basic nursing intervention on cerebral ischemia-reperfusion injury in rats and its effects on the expression of apoptosis-related proteins Bcl-2, Bax and caspase-3. The mNSS score, brain index, brain water content, infarction volumes, MDA activity, and the levels of IL-6, IL-8, TNF-α and IL-1β as well as the protein levels of p-53, NF-κB, Bax and Caspase-3 were significantly increased, while the levels of Bcl-2 protein, SOD, GSH-Px and T-AOC were significantly decreased in I/R group. These levels were reversed in SA group, PNS group and SA + PNS group. SA, PNS and they combined with basic nursing have protective effects on cerebral I/R injury, and the combination with basic nursing has better effects than that used alone. e mechanism may be to regulate the expression of downstream apoptotic proteins by inhibiting the TLR4/NF-κB signaling pathway, thereby reducing neurological damage in rats

Introduction

Materials and Methods

Results

Findings

Discussion