Clinical manifestation of norovirus infection in children aged less than five years old admitted with acute diarrhea in Surabaya, Indonesia: a cross-sectional study.

Alpha Fardah Athiyyah,Koichi Kitagawa,Andy Darma,Nazara Agustina,Reza Ranuh,Subijanto Marto Sudarmo,Toshiro Shirakawa,Katsumi Shigemura,Dadik Raharjo,Masato Fujisawa

doi:10.12688/f1000research.21069.3

Alpha Fardah Athiyyah, Koichi Kitagawa + Show 8 more

Open Access

https://doi.org/10.12688/f1000research.21069.3

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Abstract

Background: The objective of this study was to investigate the clinical manifestation of norovirus infection between norovirus genogroup and severity of acute diarrhea in pediatric patients at the Dr. Soetomo Hospital, Surabaya, Indonesia. Methods: This cross-sectional study involved 31 participants aged 1-60 months admitted to the hospital with acute diarrhea from April 2012 to March 2013. Norovirus genogroups (GI and II) were identified from patient stool using reverse transcription polymerase chain reaction (RT-PCR). Severity was measured using the Ruuska and Vesikari scoring system. Results: In total, 94 stool samples were obtained, of which 31 (19%) were norovirus positive. Norovirus GI was found in one sample with mild diarrhea. Norovirus GII was found in 30 samples (96.8%); one sample with mild diarrhea (3.3%), 20 samples with moderate diarrhea (66.7%), and nine samples with severe diarrhea (30%). Conclusion: Norovirus GII was the most prevalent cause of acute diarrhea and 30% of the cases manifested as severe diarrhea.

Highlights

The objective of this study was to investigate the clinical manifestation of norovirus infection between norovirus genogroup and severity of acute diarrhea in pediatric patients at the Dr Soetomo Hospital, Surabaya, Indonesia
This study aimed to examine the clinical manifestation of norovirus infection in pediatric patients aged 1–60 months in the Dr Soetomo General Hospital, Surabaya, Indonesia
Most of the participants whose samples were positive for norovirus were male (54.8%), the youngest participant was one month old and the oldest was 24 months old

Summary

Introduction

The objective of this study was to investigate the clinical manifestation of norovirus infection between norovirus genogroup and severity of acute diarrhea in pediatric patients at the Dr Soetomo Hospital, Surabaya, Indonesia. Methods: This cross-sectional study involved 31 participants aged 160 months admitted to the hospital with acute diarrhea from April 2012 to March 2013. Norovirus genogroups (GI and II) were identified from patient stool using reverse transcription polymerase chain reaction (RT-PCR). Severity was measured using the Ruuska and Vesikari scoring system. Results: In total, 94 stool samples were obtained, of which 31 (19%) were norovirus positive. Norovirus GI was found in one sample with version 3 (revision)

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