Abstract
At the Summit on Organ Banking through Converging Technologies held recently in Boston, tissue and organ cryopreservation technology was a topic of considerable interest. Although cryopreservation has been widely used in clinical practice, it currently remains limited to bloodless tissues with simple structures and functions that are small or thin, for example, ultra-thin skin, ovarian tissue slices, and other similar tissues. For whole organs, except for successful cryopreservation of rat ovaries (2002) and hind limbs (August 2002), successful cryopreservation of vascularized animal tissues or organs and their replantation have not yet been reported. We conducted histological and electron microscopic examinations on muscle after blood supply restoration to explain this problem and describe our experience with the goal of informing our colleagues to further develop the technology. To achieve broad application of vascularized tissue and organ cryopreservation, we have summarized our experience and established a clinical application scope for vascularized composite tissue cryopreservation.
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