Abstract

Ionized magnesium was measured using a clinical analyzer MICROLYTE 6 (KONE, Finland). Total magnesium was measured colorimetrically. The influence of anion-ligands on Mg-ISE responses was investigated. Ionized magnesium (iMg) concentration changes with pH were made to observe the accuracy of results. The dependence of iMg and iCa on heparin (used as anticoagulant in plasma samples) was made. Less than 40 units per 1 ml of blood decreased the concentration of iMg and iCa less than 1.5%. The range of iMg and total Mg in serum and plasma of healthy and ill adults (short bowel syndrome, myocardial infarct) was investigated and compared to each other. In both illnesses, the concentration of ionized and/or total magnesium is significantly different from the range for healthy people. The range of iMg and total Mg as well as the percentage of iMg against total Mg seem to be specific for given illness. The range of iMg and total Mg in blood serum of healthy children was also investigated.

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