Clinical evaluation of the Magicplex Sepsis Real-time Test (Seegene) to detect Candida DNA in pediatric patients

J Serra,Jl Dapena,C Figueras,Y Peña,E Rosello,C Díaz-Heredia,A Andreu,M Pujol,Mg Codina,P Céspedes

doi:10.1186/cc11729

Abstract

Clinical evaluation of the Magicplex Sepsis Real-time Test (Seegene) to detect Candida DNA in pediatric patients

Highlights

Central venous catheterization (CVC) is a frequently performed procedure in ICUs for both monitoring and definitive central venous access
A functional FCGR2A polymorphism leading to amino acid change of histidine (H) to arginine (R) at position 131 appears to be a major candidate in adult invasive pneumococcal diseases (IPD)
Our study revealed no significant difference between the low-tidal volume ventilation and the traditional strategy ventilation groups in mean (± SD) ventilator-free days (12 ± 9 vs. 11 ± 8, P = non significant) respectively), the number of days without ventilator use during the first 28 days after randomization was greater in the low-tidal volume group

Summary

Introduction

Central venous catheterization (CVC) is a frequently performed procedure in ICUs for both monitoring and definitive central venous access. Conclusion: GAPDH mRNA expression in patients with severe sepsis showed a marked increase compared with controls These data question the suitability of GAPDH as a housekeeper gene in gene expression profiling studies in sepsis. Cytokine gene expression profiling using the Quantigene plex assay is able to demonstrate distinct profiles in patients with severe sepsis This has the potential to be developed into a diagnostic/prognostic tool with larger studies. Results: In all studies we observed that elevated blood levels of ESM-1 correlated with the severity of sepsis and the poor outcome in patients with severe sepsis or in septic shock at ICU admission. In a context where respiratory failure is still the first cause of death in sepsis, our study analysis suggests that blood levels of ESM-1 may be a useful early biomarker of lung tissue injury and respiratory failure in ICU patients. Blood culture resulted positive in 40.6% of patients with sepsis

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