Abstract
P. KrÖll's modification (1951) was electrophotometrically reexamined and found more sensitive than original Manoiloff's colour-reaction which is thought as being a test to indicate the various state of the neurovegetative tonus. The reaction is ruled by Lambert-Beer's law and the filter 610μ is most suitable for its estimation. While pH of reagents exeats a far-reaching influence upon this colouration, both storage of blood sample within 20° and temperature from 4 to 37°C have no significant effect upon T% in modified Manoiloff's reaction. Observing the mode of discolouration, it is found that the proper time for the estimation is from 30' to 60' after the administeration of reagents. Although it can not be yet concluded decidedly to which serum fraction the water-blue has affinity, the protective action of the serum on proteine (globuline) is taken into account, agreeing with Kröll, concerning the chemism of this reaction which can not be much related to pH of the test serum itself.T% of the blood of 30 normal cases examined under the same condition shows froms 67 to 83 (mean value %73.9; 77.95≥m≥69.85). Simultaneous observations of other autonomic function tests including the effects of serveral drugs on the authonomic nervous system were studied on patients suffering from various kinds of vegetative disorders and proved that Manoiloff-Kröll's reaction corresponds to much extent to the autonomic nervous state despite of some exception; details were discussed in each case.
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