Abstract
Respiratory viruses are among the most important causes of human morbidity and mortality worldwide, especially for infants and young children. In the past years, a few commercial multiplex RT-PCR assays have been used to detect respiratory viruses in spite of the high cost. In the present study, an improved single-tube multiplex reverse transcription PCR assay for simultaneous detection of 13 respiratory viruses was evaluated and compared with a previously reported two-tube assay as the reference method using clinical nasopharyngeal aspirates samples. Of 310 prospectively tested respiratory specimens selected from children hospitalized with acute respiratory illness, 226 (72.90%, 226/310) and 214 (69.03%, 214/310) positive for one or more viruses were identified by the single-tube and the two-tube assays, respectively, with combined test results showing good concordance (Kappa value = 0.874). Individually, the single-tube assay for adenovirus (Adv), human metapneumovirus (HMPV), human rhinovirus (HRV), parainfluenza virus type 1 (PIV1), parainfluenza virus type 3 (PIV3) and parainfluenza virus type 4 (PIV4) showed the significantly superior sensitivities to those of the two-tube assay. No false positives were found. In conclusion, our results demonstrates the one-tube assay revealed significant improvements over the two-tube assay in terms of the better sensitivity, more accurate quality control, less nonspecific amplification, more cost-effective and shorter turn-around time and will be a valuable tool for routine surveillance of respiratory virus infection in China.
Highlights
Respiratory viruses are among the most common causes of human morbidity and mortality worldwide, especially for infants and young children [1, 2]
The aim of this study is to provide a high throughput screening method for routine surveillance of respiratory virus infection in provincial and local Centers for Disease Control and Prevention, China
Clinical specimens were obtained from 310 hospitalized patients who had acute respiratory infection(ARI) and admitted to the children's hospital of Hebei, China bet-ween May-October, 2015
Summary
Respiratory viruses are among the most common causes of human morbidity and mortality worldwide, especially for infants and young children [1, 2]. In the last few years, multiplex RT-PCR assays have been developed to detect respiratory viruses, and many of them have been commercialized, such as xTAG RVP, RVP fast (Luminex Molecular Diagnostics, Toronto, Canada), Resplex II (Qiagen, Mississauga, Canada), FilmArray Respiratory panel (Idaho Technology Inc., Salt Lake City, UT, USA), AnyplexTM II RV16 and Seeplex RV assays (Seegene, seoul, Korea), AdvanSureTM real-time RT-PCR (LG Life Science, Seoul, Korea) [3,4,5,6,7]. Many of these assays are costly, or require specialized laboratory equipment [8, 9]
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