Abstract
Article1 July 1940CLINICAL APPLICATION OF THE THIOCHROME REACTION IN THE STUDY OF THIAMIN (VITAMIN B1) DEFICIENCYHARRY J. BORSOX, M.D.HARRY J. BORSOX, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-14-1-1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptA Chemical method for the estimation of thiamin (vitamin B1), should have the advantages, as compared to biological assay, of speed, accuracy, cheapness, and ease of performance. The great advantage of biological assay is definite specificity. However, under well controlled conditions the uncertainties of chemical assay may become negligible.Of the several methods proposed for the chemical estimation of thiamin, the thiochrome method of Jansen1has received the most attention. Recently, the method of Prebluda and McCollum2as modified by Melnick and Field3has been shown to possess a high degree of specificity and accuracy, but the technic as described...
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