Abstract
Objective To analyse the relationship between lymphatic vessels invasion and clinical pathological features of papillary thyroid carcinoma(PTC). Methods The expressions of D2-40 and CK19 were examined in the 104 specimens of PTC using immunohistochemical staining with combined monoclonal antibodies and cocktail double enzyme labeled antibody(D2-40/CK19) stainings. The two methods were compared in the diagnosis of PTC metastasis, and the factors affecting lymphatic vessels formation were analyzed. Results The positive rate of lymphatic vessels invasion was 37.5%(39/104) by using immunohistochemical staining with combined monoclonal antibodies and 53.8%(56/104) by cocktail double enzyme labeled antibody(D2-40/CK19) staining(P<0.05). The lymph node metastasis rate was 83.9%(47/56) in the group with lymphatic vessels invasion, significantly higher than that without invasion 22.9%(11/48, P<0.01). The age of patients, diameter of primary tumor were the influence factors of lymphatic vessels invasion in PTC patients(P<0.05 and P=0.063). Conclusion Cocktail double enzyme labeled antibody(D2-40/CK19) staining is a better method to detect lymphatic vessels invasion in PTC than immunohistochemical staining with combined monoclonal antibodies. (Chin J Endocrinol Metab, 2016, 32: 107-111) Key words: Lymphatic vessel invasion; Cocktail double enzyme labeled antibody staining; CK19; D2-40; Papillary thyroid carcinoma
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