Clinical application of acellular matrix derived from the bubaline diaphragm and caprine rumen for the repair of abdominal wall defects in animals

Harendra S Rathore,Naveen Kumar,Ravi K Agarwal,Swapan K Maiti,Kiranjeet Singh,Shivaraju Shivaramu,Sameer Shrivastava,Sonal Saxena

doi:10.13170/ajas.4.2.13071

Harendra S Rathore, Naveen Kumar + Show 6 more

Open Access

https://doi.org/10.13170/ajas.4.2.13071

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Abstract

The abdominal wall hernias resulting due to trauma or other clinical conditions are common in animals. Large hernias required the use of synthetic mesh, which is costly and may result in infection, fistula formation, and pain. Application of biomaterials in hernia repair causes a reduction in pain, reduced recovery time, and rate of recurrence. The study was undertaken to test the acellular bubaline diaphragm matrix (BDiaM) and acellular caprine rumen matrix (CRuM) for the repaired hernia in clinical cases. Fresh bubaline diaphragm and caprine rumen were decellularized using sodium deoxycholate (1% for CRuM and 2% for BDiaM) for 48h. Acellularity was ascertained histologically and by DNA quantification. Histologically, both the matrices showed complete acellularity and orderly arranged collagen fibers after 48 h. The DNA contents were significantly (P0.05) reduced in both the matrices in comparison to the native matrices. The BDiaM and CRuM matrices were applied in eight and nine clinical cases of abdominal wall defects, respectively. Animals with BDiaM and CRuM matrices recovered uneventfully and remained sound at least up to 3 months. Hematological and immunological findings were unremarkable. BDiaM and CRuM matrices showed good results without complications. Keywords:Biocompatibility, Bubaline diaphragm matrix, Caprine rumen matrix, DNA quantification, ELISA, SDS-PAGE

Highlights

The abdominal wall defects due to trauma in animals are quite common (Ladurner et al, 2001)
The abdominal wall hernias resulting due to trauma or other clinical conditions are common in animals
Large hernias required the use of synthetic mesh, which is costly and may result in infection, fistula formation, and pain

Summary

Introduction

The abdominal wall defects due to trauma in animals are quite common (Ladurner et al, 2001). Primary closure can repair small abdominal wall defects while hernias with massive defects, including irreducible hernia, requires special surgical procedures by the use of graft (Iqbal et al, 1994). The synthetic mesh may cause infection, fistula formation, and pain. Use of biomaterials in hernia repair causes a reduction in pain, less recovery time, and reduced rate of recurrence (Amid, 1997). Synthetic non-absorbable meshes provide good mechanical strength but may cause intestinal adhesions and obstruction, wound infection, fistula formation, and seroma/hematoma formation (Eid et al, 2003). Biological materials provided a framework for fibroblast proliferation and minimized adhesion formation (Clarke et al, 1996).

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