CLINICAL AND MOLECULAR REPORT OF A RARE RAM POSITIVE NON-DOWN SYNDROME INFANT CBFA2T3-GLIS2 NEGATIVE, PRESENTING THE DRIVER P210-BCR-ABL1

Abstract

Pediatric Acute Megakaryoblastic Leukemia (AMKL) comprises 4%‒15% of Acute Myeloid Leukemia (AML) cases. Down Syndrome (DS)-AMKL usually displays a favorable outcome, whereas the prognosis of non-DS-AMKL is generally poor. Here we present the clinical and genomic data of a rare non-DS-AMKL infant with p210-BCR-ABL1 fusion, co-occurring with a high-risk WT1 mutation. The baby was RAM positive and CBFA2T3-GLIS2 negative, showing refractoriness to treatment. A 1-year-old girl was referred to the Hospital with a history of fever and motor developmental delay for four months. On admission, she presented ecchymosis, swelling, and organomegaly (spleen and liver). The WBC count was 26.5 × 10 3 /μL, hemoglobin level was 7.6 g/dL, platelet count was 25 × 10 3 /μL, and LDH was 2.887. At diagnosis, she showed bilateral myeloid sarcoma. Morphological analysis of the Bone Marrow (BM) showed clustered cytoplasmic blebs. Flow cytometry analysis revealed 83% of blast cells and a profile compatible with AMKL and RAM phenotype. The patient was treated under the high-risk AML-BFM-2004. Imatinib was incorporated into the treatment after BCR-ABL1 detection. However, minimal residual disease remained positive throughout the treatment, making BM transplantation unfeasible. Disease refractoriness was persistent even with the administration of Dasatinib, FLAG-IDA and Venetoclax. The patient died one year after diagnosis due to sepsis during BM aplasia. We performed G-banding, FISH, and Multiplex RT-PCR experiments. The molecular response was assessed by absolute quantification via RT-qPCR assays. Sanger sequencing and NGS were performed on a targeted approach. The karyotype was 46,XX,t(9;22)(q21;q34). We observed polyploidy (3‒20N). FISH in polyploid Nuclei (8N) showed four BCR-ABL1 fusion signals. In diploid metaphases, we observed two BCR-ABL1 signals. Multiplex RT-PCR detected the p210- BCR - ABL1 . RT-qPCR monitoring detected persistent high levels of BCR-ABL1 transcripts associated with disease refractoriness. Sanger sequencing showed no GATA1 mutation. NGS revealed a missense WT1 pathogenic mutation, and CBFA2T3-GLIS2 was not present. RAM phenotype is characterized by overexpression of CD56 and under-expression of CD38, CD45, and HLA-DR. This phenotype is characteristic of children, usually associated with AML-M7 (38%) and intermediate-risk cytogenetics not associated with prognostic subgroups. RAM is related to a poor prognosis with high MRD and induction failure rates compared to non-RAM patients. This phenotype is associated with the CBFA2T3-GLIS2 fusion, which is related to extramedullary involvement, a particular blast morphology, and a worse outcome as compared to fusion-negative AML pediatric patients, including a high frequency of disease refractoriness and relapse. NGS studies did not show CBFA2T3-GLIS2 in our patient. Thus, our results indicate that RAM+ associated with the driver p210-BCR-ABL1, even with specific targeted therapy, may lead to treatment resistance regardless of CBFA2T3-GLIS2+. Here we highlight for the first time the presence of p210-BCR-ABL1 associated with RAM phenotype in the absence of CBFA2T3-GLIS2 and disease refractoriness in a non-DS-AMKL infant.