Clinical and immunological features of activation of latent herpesvirus infection in children with rotavirus infection

Abstract

The analysis of clinical parameters and indicators of immune response in rotavirus gastroenteritis in children aged 12 to 36 months infected with herpes viruses was performed. Verification of the diagnosis of RVI was carried out by isolating rotavirus antigen from the feces of patients by enzyme-linked immunosorbent assay (ELISA) and the corresponding IgM antibodies from the blood. Also, all children were examined for herpes virus infection types 1, 2, 4, 5, 6. The level of specific IgG and IgM to these herpesviruses was determined by ELISA. In the case of a positive result, the presence of DNA (qualitatively) was determined by polymerase chain reaction (PCR). PCR blood tests for the presence of herpesvirus DNA were performed twice, in the acute period of RVI and in the period of early convalescence. The first group included 71 children in whom rotavirus gastroenteritis occurred on the background of latent herpesvirus infection, in the second ¬ 15 children in whom during the early convalescence there was a reactivation of herpesvirus infection. The obtained data indicate that the activation of herpesvirus infection occurs against the background of reduced immunoregulatory index in the acute period of the disease, and in the period of early convalescence RVI is characterized by the preservation of elevated CD 16+ cells and proinflammatory cytokines (IL-1β and TNF-α). in our opinion, leads to a prolongation of clinical symptoms of rotavirus infection. Keywords: children, rotavirus infection, latent and active herpesvirus infection, cellular and humoral immunity, cytokines.