Clinical and immunological differences between asymptomatic HDM-sensitized and HDM-allergic rhinitis patients.

Abstract

Confirmation of the clinical relevance of sensitisation is important for the diagnosis of allergic rhinitis. To investigate the usefulness of an in vitro basophil activation test and component-resolved diagnosis in distinguishing between symptomatic allergic rhinitis patients and asymptomatic sensitization to house dust mites (HDMs). Thirty-six subjects with a positive skin prick test (SPT) for HDM were divided into a symptomatic (n=17) and an asymptomatic (n=19) group on the basis of their clinical history and a nasal provocation test. A basophil CD63 response to in vitro stimulation with Dermatophagoidespteronyssinus whole allergen extract and the IgE reactivity profiles for Der p 1, 2, 4, 5, 7, 10, 11, 14, 15, 18, 21, 23 were evaluated. Serum IgE and IgG specific to Dpteronyssinus whole allergen extract and total IgE were measured. There were no statistically significant differences in the levels of IgE (IgE levels were higher in symptomatic patients with P=0.055) and IgG specific to Dpteronyssinus and total IgE. Symptomatic patients showed a lower threshold for in vitro basophil activation (3.33ng/mL vs 33.3ng/mL), a higher area under the curve (AUC) of basophil activation (171 vs 127) (P=0.017), a higher response to positive control with anti-FcεRI stimulation (97% vs 79%) (P<0.001), a recognition of more HDM allergens (4 vs 2) and more frequent sensitization to rDer p 7 (P=0.016) and rDer p 23 compared to asymptomatic subjects (P=0.018). There was a positive correlation (r=0.63; P<0.001) between the number of recognized allergens and the AUC of basophil activation. In the subjects studied, the differences in the basophil response to Dpteronyssinus allergen extract, number of recognized HDM allergens and reactivity to rDer p 7 and rDer p 23 distinguish symptomatic from asymptomatic HDM sensitisation better than SPT or allergen extract-specific IgE. Information regarding the clinical relevance of sensitization is important for the prescription of allergen-specific immunotherapy.