Abstract
目的分析9例遗传性凝血因子Ⅴ(FⅤ)缺乏症患者的临床表现及分子致病机制。方法对1999年4月至2019年9月就诊于中国医学科学院血液病医院的9例遗传性FⅤ缺乏症患者进行回顾性分析:应用活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)及FⅤ促凝活性(FⅤ∶C)测定进行表型诊断;使用高通量靶向测序筛查F5基因变异,Sanger测序验证并分析双亲携带情况;Swiss-model进行三维结构分析,ClustalX-2.1软件进行同源保守性分析。结果9例患者的FⅤ∶C为0.1~10.6 U/dl,其中8例患者有出血病史,以皮肤/黏膜出血最为多见(3例),其余1例未发生出血事件。所有患者中纯合子5例,复合杂合子4例,共检测到12个致病或疑似致病F5基因突变,其中c.6100C>A/p.Pro2034Thr、c.6575T>C/p.Phe2192Ser、c.1600_1601delinsTG/p.Gln534*、c.4713C>A/p.Tyr1571*和c.952+5G>C为首次报道。结论该研究新发现的基因突变丰富了与遗传性FⅤ缺乏症相关F5基因突变谱,高通量测序方法可以有效检测F5基因突变。
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