Clinical and Functional Relevance of Melanocortin-4 Receptor Variants in Obese German Children

Abstract

Background: Variants in the melanocortin-4 receptor (MC4R) gene are the most frequent cause of monogenic obesity. The relevance of MC4R variations with respect to clinical phenotype and biochemical function remains controversial. Methods: We sequenced the MC4R gene in 510 overweight/obese children. The clinical phenotype was assessed in a case-control setting matched for age, gender, puberty and body mass index. Identified MC4R variants were functionally characterized in vitro. Results: The frequency of MC4R variants was 5.3%, with functionally relevant mutations (D⁹⁰N, V¹⁰³I/S¹²⁷L, R¹⁶⁵W, G¹⁸¹D) occurring in 1.2% (confidence interval 0.26–2.15) of our sample. 4.1% were carriers of variants (Y³⁵Y, V¹⁰³I, T¹¹²M, M²⁰⁰V, I²⁵¹L) with preserved receptor function in vitro. We did not detect large heterozygous deletions by multiple-ligand probe amplification assay. There were no differences in anthropometric or metabolic parameters between children with loss-of-function mutations and noncarriers. Carriers of the V¹⁰³I or I²⁵¹L variant had higher high-density lipoprotein cholesterol and HbA1c levels than matched noncarriers of MC4R variants. Conclusions: In our data set of childhood obesity in central Germany, we identified functionally relevant mutations in the MC4R gene in only 1.2% of the children. There were no major significant phenotypic differences between obese children with and without MC4R mutations. Hence, the diagnosis of genetically caused obesity due to MC4R mutation should be made with caution.