Abstract
To study the effect of Cleistocalyx nervosum extract (CE) on diethylnitrosamine (DEN) and phenobarbital (PB) induced oxidative stress in early stages of rat hepatocarcinogenesis. Male Wistar rats were divided into 4 groups, with Group 1 as a negative control and Group 2 was a positive control receiving DEN injections once a week and PB in drinking water for 6 weeks. Two weeks before DEN initiation and PB treatment, Groups 3 and 4, were fed with 500 and 1000 mg/kg of CEs, respectively, for 8 weeks. A number of GST-P-positive foci, preneoplastic lesions, in the liver were markedly increased in carcinogen administered rats, but was comparatively decreased in rats treated with 1000 mg/kg of CE. The CE reduced malondialdehyde in serum and in the livers of rats treated with DEN and PB. Moreover, CE significantly increased the activities of glutathione peroxidase and catalase in rat liver. CE appeared to exert its chemopreventive effects by modulating antioxidant status during DEN and PB induced early stages of hepatocarcinogenesis in rats.
Highlights
Oxidative stress has long been known to be involved in the pathogenesis of age-related and chronic diseases, including cancer
We investigated the effect of C nervosum extract on DEN and PB induced oxidative stress during the early stages of hepatocarcinogenesis in rats, using Glutathione-S-transferase placental form (GST-P) positive foci as the endpoint marker
Effect of C nervosum extract on lipid peroxidation and antioxidant enzymes in liver and serum of rats treated with diethylnitrosamine and phenobarbital
Summary
Oxidative stress has long been known to be involved in the pathogenesis of age-related and chronic diseases, including cancer. It is defined as an imbalance between oxidants and antioxidants in favor of the former, resulting in an overall increase in cellular levels of reactive oxygen species (ROS) (Klaunig and Kamendulis, 2004). The detection of GST-P-positive hepatocytes is one important tool for analyzing relevant carcinogenic or anticarcinogenic responses during the initiation and promotion stages of rat liver carcinogenesis (Ito et al, 1988). We investigated the effect of C nervosum extract on DEN and PB induced oxidative stress during the early stages of hepatocarcinogenesis in rats, using GST-P positive foci as the endpoint marker
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