Abstract
The purpose of my project is understanding the specific amino acid interactions for the B‐cell Receptor, BAFF‐R, protein interacting with a RNA aptamer. A RNA aptamer is a peptide that binds to a molecule of interest. We had to express our protein with a GST tag. We used different lab skills, such as gel shift assay, to investigate the interaction between the BAFF‐R and its RNA aptamer. However, the results were inconclusive as we saw an unexpected binding pattern. We hypothesize that cleaving the Glutathione S‐Transferase (GST) tag using thrombin will provide for our expected binding pattern in an EMSA.Support or Funding InformationFunded by Office of Undergraduate Research in Sonoma State University.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
