Claustral efferents to the cat's limbic cortex studied with retrograde and anterograde tracing techniques

Abstract

The claustral projections to the cat's limbic cortex were investigated with the horseradish peroxidase retrograde tracing technique and with autoradiography. Autoradiographic injections covered small portions of either the dorsal anterior claustrum or intermediate to posterior regions of the claustrum. Injections of horseradish peroxidase were made into the subicular, insular, entorhinal, prepiriform, cingulate, retrosplenial and prefrontal cortex. Both methods revealed fully consistent data for substantial claustral efferents to the cingulate, retrosplenial, entorhinal and subicular cortex. For the prepiriform cortex claustral efferents could be established unequivocally only with the horseradish peroxidase technique. Only a rather minor projection could be traced for the claustro-insular projection. Unilateral injections of horseradish peroxidase revealed the existence of a minor number of labeled claustral cells in the contralateral hemisphere for all loci except insular and prepiriform ones. Our data show that claustral cells reach the majority of the allocortical areas of the brain. They thereby confirm the view that the claustrum projects to most regions of the cortex and furthermore that a certain kind of topography exists in the claustro-cortical afferents with a minor number of claustral cells sending afferents to the contralateral cortical hemisphere. In addition, our data reveal that the distribution of claustrocortical afferents is uneven and that the ventral claustrum (or nucleus endopiriformis) sends fibers to more cortical regions than previously assumed. It is suggested that the claustrum participates in the integration of sensory, motivational, emotional and mnemonic information via its reciprocal claustro-neocortical and its claustro-limbic connections.