Classification of Streptomyces with the V2 variable region in 16S rDNA

Abstract

Partial nucleotide sequences (120 bp) of the 16S rRNA gene (rDNA) containing V 2 variable region were compared in 357 strains of Streptomyces . Most of the Streptomyces strains fell into one large cluster group. By sharing the same 16S rDNA sequences or very high similarity, they were clustered into five major, three middle and eight minor clusters, and 19 strains recovered as single member clusters. The result demonstrated that the phenotypic clusters, which contains strains that are different in terms of morphologic characteristics, such as spore color and spore surface, is very heterogeneous from a molecular systematic stand point.