Abstract
Green pea peroxidase isoenzymes were partially purified, identified, and grouped by preparative isoelectric focusing in granulated gel (PEGG), using a modified cascade method. Isoenzyme patterns were detected utilizing a substrate-impregnated paper print technique. Direct application of crude extract on PEGG proved to be a valuable tool for partial purification of peroxidase isoenzymes. A substrate-impregnated paper print technique enabled the detection of isoenzyme patterns and may replace the time-consuming conventional peroxidase activity assay. Twelve isoenzymes (pIs ranging from 5.5–8.6), isolated on analytical thin-layer isoelectric focusing on polyacrylamide gel, were isoelectrically homogeneous.
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