Classification of ginseng berry (Panax ginseng C.A. MEYER) extract using 1H NMR spectroscopy and its inhibition of lipid accumulation in 3T3-L1 cells.

Seung Ok Yang,Hyung-Kyoon Choi,Hae Ran Park,Young Ock Kim,Sang Won Lee,Sae Won Na,Young Chang Kim,Hyung Don Kim,Kee Hong Kim,Eun Suk Sohn,Mariadhas Valan Arasu

doi:10.1186/1472-6882-14-455

Abstract

BackgroundPanax ginseng is a famous traditional medicine in Korea for its beneficial effect on obesity, cardiac and liver associated diseases. The aim of this study was to investigate the metabolite in Panax ginseng (P. ginseng, Aralicaceae) berries depending on the ripen stages and evaluate its potential inhibition on adipocyte differentiation in 3 T3-L1 cells.MethodsDifferent ripening stage samples of P. ginseng berry were analyzed through global metabolite profiling by NMR spectroscopy. Lipid accumulation in the cells was analyzed by Oil Red O staining.ResultsThe PLS-DA clearly distinguished P. ginseng berry extract (PGBE) according to the partial ripe (PR), ripe(R) and fully ripe (FR) stage. Lipid accumulation of PGBE was examined by measuring triglyceride content and Oil-Red O staining. These results suggested that the FR stage of PGBE decrease in lipid accumulation during adipocyte differentiation and the amount of threonine, asparagine, fumarate, tyraine, tyrosine, and phenylalanine increased with longer ripening of ginseng berries.ConclusionMetabolite profiling of P. ginseng was identified by 1H NMR spectra. P. ginseng extract efficiently inhibits adipogenesis in 3 T3-L1 adipocytes concluded that the P. ginseng has the antiobesity properties.

Highlights

Panax ginseng is a famous traditional medicine in Korea for its beneficial effect on obesity, cardiac and liver associated diseases
We have looked for lipid accumulation in adipocyte inhibitory plants using P. ginseng berry extract (PGBE) as an in vitro assay system
Oil Red O staining Lipid accumulation of PGBE was examined by measuring triglyceride content using Oil-Red O staining

Summary

Introduction

Panax ginseng is a famous traditional medicine in Korea for its beneficial effect on obesity, cardiac and liver associated diseases. Obesity is a risk factor for major metabolic disease including type 2 diabetes, atherosclerosis, hyperlipidemia and hypertension and multifactorial syndrome in human [1,2]. It is an abnormal condition in which the lipids are accumulated in adipose tissues and various kinds of adipokine [3]. P. ginseng root is normally harvested between the fourth and sixth year of growth. P. ginseng berry be harvested several times after the third year of growth [10]. Quan et al [11] reported that

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