Classification of caudal ventrolateral pontine neurons with sympathetic nerve-related activity.

Abstract

This study was designed to answer three questions concerning caudal ventrolateral pontine (CVLP) neurons whose naturally occurring discharges are correlated to sympathetic nerve discharge (SND). 1) What are the proportions of CVLP neurons that have activity correlated to both the cardiac-related and 10-Hz rhythms in SND, to only the 10-Hz rhythm, and to only the cardiac-related rhythm? 2) Do CVLP neurons with activity correlated to the cardiac-related and/or 10-Hz rhythm in SND subserve a sympathoexcitatory or sympathoinhibitory function? 3) Do CVLP neurons with activity correlated to the cardiac-related and/or 10-Hz rhythm in SND project to the thoracic spinal cord? To address these issues we recorded from 476 CVLP neurons in 24 urethan-anesthetized cats. Spike-triggered averaging, arterial pulse-triggered analysis, and coherence analysis revealed that the discharges of 66 of these neurons were correlated to inferior cardiac postganglionic SND. For 39 of these neurons, we were able to determine whether their discharges were correlated to one or both rhythms. The results showed that the CVLP contained a heterogeneous population of neurons with sympathetic nerve-related activity. The discharges of 21 neurons were correlated to both the 10-Hz and cardiac-related rhythms in SND, 9 neurons had activity correlated to only the 10-Hz rhythm, and 9 neurons had activity correlated to only the cardiac-related rhythm. The firing rates of CVLP neurons with activity correlated to both rhythms or to only the 10-Hz rhythm were decreased during the inhibition of SND induced by baroreceptor reflex activation (rapid obstruction of the abdominal aorta). These neurons are presumed to exert sympathoexcitatory actions. The time-controlled collision test verified that 11 of 12 CVLP neurons with activity correlated to both rhythms were antidromically activated by stimulation of the first thoracic segment of the spinal cord. Antidromic mapping at this level showed that the site requiring the least stimulus current to elicit the longest latency response (nearest the terminal) was in the vicinity of the intermediolateral nucleus (IML). In contrast, only 1 of 13 CVLP neurons with activity correlated to only one of the rhythms in SND could be antidromically activated by spinal stimulation. These data demonstrate for the first time that there is a direct pathway from the CVLP to the IML that is comprised almost exclusively of sympathoexcitatory neurons whose discharges are correlated to both the 10-Hz and cardiac-related rhythms in SND.