Classification, identification, and growth stage estimation of microalgae based on transmission hyperspectral microscopic imaging and machine learning.

Abstract

A transmission hyperspectral microscopic imager (THMI) that utilizes machine learning algorithms for hyperspectral detection of microalgae is presented. The THMI system has excellent performance with spatial and spectral resolutions of 4 µm and 3 nm, respectively. We performed hyperspectral imaging (HSI) of three species of microalgae to verify their absorption characteristics. Transmission spectra were analyzed using principal component analysis (PCA) and peak ratio algorithms for dimensionality reduction and feature extraction, and a support vector machine (SVM) model was used for classification. The average accuracy, sensitivity and specificity to distinguish one species from the other two species were found to be 94.4%, 94.4% and 97.2%, respectively. A species identification experiment for a group of mixed microalgae in solution demonstrates the usability of the classification method. Using a random forest (RF) model, the growth stage in a phaeocystis growth cycle cultivated under laboratory conditions was predicted with an accuracy of 98.1%, indicating the feasibility to evaluate the growth state of microalgae through their transmission spectra. Experimental results show that the THMI system has the capability for classification, identification and growth stage estimation of microalgae, with strong potential for in-situ marine environmental monitoring and early warning detection applications.