Abstract
Classical Genetics Meets Next-Generation Sequencing: Uncovering a Genome-Wide Recombination Map in Drosophila melanogaster
Highlights
Homologous recombination is a potent genetic force that impacts myriad aspects of genome evolution, from standing levels of nucleotide diversity to the efficacy of natural selection
recombinant advanced intercross lines (RAIL) females were individually crossed to D. simulans, and the genomes of single hybrid progeny were sequenced with Illumina technology
The sheer number of events catalogued combined with the resolution at which breakpoints could be mapped facilitates a great deal more than quantifying intra- and inter-genomic recombination rate variation
Summary
Homologous recombination is a potent genetic force that impacts myriad aspects of genome evolution, from standing levels of nucleotide diversity to the efficacy of natural selection. To create this landmark map, Comeron and colleagues generated recombinant advanced intercross lines (RAIL), derived from eight crosses among twelve wild-derived lines. To accurately identify crossover and noncrossover events, haplotype rather than genotype data are required, and Comeron and colleagues use a clever technique to recover haplotypes. Reads mapping to D. simulans were removed bioinformatically to reveal a haploid, meiotically produced D. melanogaster genome.
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