Classical conditioning of the rabbit's nictitating membrane response under fixed and mixed CS-US intervals

Abstract

The rabbit's nictitating membrane response was classically conditioned to a tone CS, followed by an electric shock US, at two randomly alternating CS-US interstimulus intervals (ISIs). Different groups were exposed to different proportions of 200- and 700-msec ISIs. The study revealed: (a) CR percentage was a direct function of the proportion of 200-msec ISIs; (b) trials to the first test-trial CR were an inverse function of the proportion of 200-msec ISIs; (c) CR onset latencies decreased over training; (d) CR peak latencies coincided with the temporal loci of the US; and (e) groups exposed to both ISIs acquired double-peaked CRs. Moreover, the amplitudes of nictitating membrane responses in the present experiment paralleled certain quantitative aspects of the free operant key peck response rates of pigeons trained under two mixed inter-reinforcement intervals. The results were discussed with respect to the micromolar response shaping and macromolecular CS-trace accounts of classical conditioning.