Abstract
BackgroundThe Campylobacter species usually causes infection between humans and livestock interaction via livestock breeding. The studies of the Campylobacter species thus far in all clinical isolates were to show the many kinds of antibiotic phenomenon that were produced. Their integrons cause the induction of antibiotic resistance between bacterial species in the Campylobacter species.ResultsThe bacterial strains from the diarrhea of pediatric patient which isolated by China Medical University Hospital storage bank. These isolates were identified by MALDI-TOF mass spectrometry. The anti-microbial susceptibility test showed that Campylobacter species resistant to cefepime, streptomycin, tobramycin and trimethoprim/sulfamethoxazole (all C. jejuni and C. coli isolates), ampicillin (89% of C. jejuni; 75% of C. coli), cefotaxime (78% of C. jejuni; 100% of C. coli), nalidixic acid (78% of C. jejuni; 100% of C. coli), tetracycline (89% of C. jejuni; 25% C. coli), ciprofloxacin (67% of C. jejuni; 50% C. coli), kanamycin (33% of C. jejuni; 75% C. coli) and the C. fetus isolate resisted to ampicillin, cefotaxime, nalidixic acid, tetracycline, ciprofloxacin, kanamycin by disc-diffusion method. The effect for ciprofloxacin and tetracycline of the Campylobacter species was tested using an E-test. The tet, erm, and integron genes were detected by PCR assay. According to the sequencing analysis (type I: dfr12-gcuF-aadA2 genes and type II: dfrA7 gene), the cassette type was identified. The most common gene cassette type (type I: 9 C. jejuni and 2 C. coli isolates; type II: 1 C. coli isolates) was found in 12 class I integrase-positive isolates.ConclusionsOur results suggested an important information in the latency of Campylobacter species with resistance genes, and irrational antimicrobial use should be concerned.
Highlights
The Campylobacter species usually causes infection between humans and livestock interaction via live‐ stock breeding
The C. jejuni, C. coli, and C. fetus strains are the major Campylobacter species in the diarrhea of pediatric patient According to the MALDI-TOF mass spectrometry results, the detection ratio of the clinical isolates for all the patient treated from January 2015 to December 2015 was 5%
71% (10/14), 21% (3/14), and 8% (1/14). These results indicate that C. jejuni, C. coli, and C. fetus strains were the major dominant Campylobacter species found in the samples from the diarrhea of pediatric patient
Summary
The Campylobacter species usually causes infection between humans and livestock interaction via live‐ stock breeding. The studies of the Campylobacter species far in all clinical isolates were to show the many kinds of antibiotic phenomenon that were produced. Their integrons cause the induction of antibiotic resistance between bacterial species in the Campylobacter species. The Campylobacter species, a bacillus, causes diseases in animals and humans [1]. Fluoroquinolones, erythromycin, and tetracycline are the first-line antibiotic agents for treatment of Campylobacter species’ infections [12]. The inappropriate use of anti-microbial agents in animal husbandry has led to the development of antibiotic resistance in Campylobacter treatment [12]. The resistance mechanisms of the Campylobacter species bacteria include horizontal gene transfer (HGT) or multidrug efflux pump [13]. The class 1 integrons associated with the aadA9 gene (aminoglycoside-resistance gene) located on an R-plasmid have been reported in Campylobacter isolates [12]
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