CLA intake from dairy fat in healthy young adults: beneficial effects on serum metabolome (643.6)

Abstract

Dairy fat is a rich source of the naturally occurring bioactive conjugated linoleic acids (CLA). We aimed to investigate the effects of dairy fat intake on serum metabolome in healthy young adults, in a CLA depletion‐repletion pilot study. Samples (n= 6) were analyzed in three sequential phases: (a) baseline; (b) at the end of an 8‐week period following a dairy fat restriction diet; and (c) at the end of an 8‐week period following a CLA‐repletion diet with regular dairy products and additional daily intake of 1.0 g of CLA from a naturally‐enriched butter. Serum metabolomic analyzes were carried out by 1H Nuclear Magnetic Resonance. Serum citrate increased in the CLA‐depletion period and returned to baseline values after CLA‐repletion (P= 0.0072; repeated measures ANOVA). Inversely, serum creatine and creatinine increased after CLA‐repletion (P< 0.05). We also observed alterations in branched‐chain aminoacids, with a tendency to decrease serum valine after CLA‐repletion. The observed changes in serum metabolites suggest a beneficial effect of dairy fat and CLA on insulin sensitivity and, possibly, on glucose homeostasis. Taken together, our results indicate that intake of natural food sources of CLA promoted beneficial changes on serum metabolic profile of healthy young adults and also demonstrated that metabolomics is an useful tool for assessing concerted metabolic effects of dairy fat intake on human health.Grant Funding Source: Supported by FAPERJ, CNPq, CAPES (Brazil)